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腾讯登录Small：开发出用纳米粒收集和再利用细胞生物酶类的新技术
| 导读 | 近日，刊登在国际杂志<em>Small</em>上的一篇研究报告中，来自克莱姆森大学的研究者在保证酶类生物活性的同时，成功地收集并且再利用了一些酶类。这些酶类是有机体产生的球蛋白类，可以增加机体生化反应的比例。
研究者Stephen表示，我们发现了简单而又强大的方法来收集吸引一些特殊的酶类，并且将其集中起来用于再利用。这些酶类在收集到之后仍然保持着其生物活性。
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近日，刊登在国际杂志<em>Small</em>上的一篇研究报告中，来自克莱姆森大学的研究者在保证酶类生物活性的同时，成功地收集并且再利用了一些酶类。这些酶类是有机体产生的球蛋白类，可以增加机体生化反应的比例。
研究者Stephen表示，我们发现了简单而又强大的方法来收集吸引一些特殊的酶类，并且将其集中起来用于再利用。这些酶类在收集到之后仍然保持着其生物活性。
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从复合物中分离出单一的蛋白质在纯化过程中是非常困难的，而且对于决定蛋白质的功能、结构和反应至关重要。研究者使用毫微型颗粒作为诱饵来捕获和再利用酶类。他们在颗粒表面设计了一种吸附酶类的靶点，允许酶类和颗粒进行结合。移去颗粒之后，酶类依然能够保持其活性。
这种诱饵颗粒的方法提供了一种分离酶类混合物的高效技术，将为生物医药领域带来新的技术。研究者建立了全面的模型来集中和提取已知的酶类配对，但是在识别未知的酶类上却并不有效。
这种新型技术也为治疗癌症埋下了伏笔，因为我们可以重点研究纳米粒的外部，来分离钝化癌症细胞生长所必须的特异酶类。演技组合目的是改变癌症关键蛋白质的细胞浓度，从而来打断癌细胞扩散的能力，因此最终控制癌症。
编译自：<a title="" href="http://www.sciencedaily.com/releases/2012/08/120808132721.htm" target="_blank">Researchers Collect and Reuse Enzymes While Maintaining Bioactivity</a>
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<img src="http://www.bioon.com/biology/UploadFiles/201208/2012080922102907.jpg" alt="" width="113" height="149" border="0" />
<a title="" href="http://dx.doi.org/doi:10.1002/smll.201200013" target="_blank">doi:10.1002/smll.201200013</a>
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<br/><strong>Substrate-Baited Nanoparticles: A Catch and Release Strategy for Enzyme Recognition and Harvesting</strong><br/>
Michael A. Daniele1, Yuriy P. Bandera1, Deepti Sharma2, Parul Rungta1, Ryan Roeder1, Michael G. Sehorn2, Stephen H. Foulger1,*
The isolation of a single type of protein from a complex mixture is vital for the characterization of the function, structure, and interactions of the protein of interest and is typically the most laborious aspect of the protein purification process. In this work, a model system is utilized to show the efficacy of synthesizing a “baited” nanoparticle to capture and recycle enzymes (proteins that catalyze chemical reactions) from crude cell lysate. Enzyme trapping and recycling is illustrated with the carbazole 1,9a-dioxygenase (CARDO) system, an enzyme important in bioremediation and natural product synthesis. The enzymes are baited with azide-modified carbazolyl moieties attached to poly(propargyl acrylate) nanoparticles through a click transformation. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis indicates the single-step procedure to immobilize the enzymes on the particles is capable of significantly concentrating the protein from raw lysate and sequestering all required components of the protein to maintain bioactivity. These results establish a universal model applicable to concentrating and extracting known substrate–protein pairs, but it can be an invaluable tool in recognizing unknown protein–ligand affinities.
<br/>来源：生物谷
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研究者Stephen表示，我们发现了简单而又强大的方法来收集吸引一些特殊的酶类，并且将其集中起来用于再利用。这些酶类在收集到之后仍然保持着其生物活性。
<!--more-->
从复合物中分离出单一的蛋白质在纯化过程中是非常困难的，而且对于决定蛋白质的功能、结构和反应至关重要。研究者使用毫微型颗粒作为诱饵来捕获和再利用酶类。他们在颗粒表面设计了一种吸附酶类的靶点，允许酶类和颗粒进行结合。移去颗粒之后，酶类依然能够保持其活性。
这种诱饵颗粒的方法提供了一种分离酶类混合物的高效技术，将为生物医药领域带来新的技术。研究者建立了全面的模型来集中和提取已知的酶类配对，但是在识别未知的酶类上却并不有效。
这种新型技术也为治疗癌症埋下了伏笔，因为我们可以重点研究纳米粒的外部，来分离钝化癌症细胞生长所必须的特异酶类。演技组合目的是改变癌症关键蛋白质的细胞浓度，从而来打断癌细胞扩散的能力，因此最终控制癌症。
编译自：<a title="" href="http://www.sciencedaily.com/releases/2012/08/120808132721.htm" target="_blank">Researchers Collect and Reuse Enzymes While Maintaining Bioactivity</a>
<div id="ztload">
<div> </div>
<div>
<div>
<img src="http://www.bioon.com/biology/UploadFiles/201208/2012080922102907.jpg" alt="" width="113" height="149" border="0" />
<a title="" href="http://dx.doi.org/doi:10.1002/smll.201200013" target="_blank">doi:10.1002/smll.201200013</a>
PMC:
PMID:
</div>
<div>
<br/><strong>Substrate-Baited Nanoparticles: A Catch and Release Strategy for Enzyme Recognition and Harvesting</strong><br/>
Michael A. Daniele1, Yuriy P. Bandera1, Deepti Sharma2, Parul Rungta1, Ryan Roeder1, Michael G. Sehorn2, Stephen H. Foulger1,*
The isolation of a single type of protein from a complex mixture is vital for the characterization of the function, structure, and interactions of the protein of interest and is typically the most laborious aspect of the protein purification process. In this work, a model system is utilized to show the efficacy of synthesizing a “baited” nanoparticle to capture and recycle enzymes (proteins that catalyze chemical reactions) from crude cell lysate. Enzyme trapping and recycling is illustrated with the carbazole 1,9a-dioxygenase (CARDO) system, an enzyme important in bioremediation and natural product synthesis. The enzymes are baited with azide-modified carbazolyl moieties attached to poly(propargyl acrylate) nanoparticles through a click transformation. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis indicates the single-step procedure to immobilize the enzymes on the particles is capable of significantly concentrating the protein from raw lysate and sequestering all required components of the protein to maintain bioactivity. These results establish a universal model applicable to concentrating and extracting known substrate–protein pairs, but it can be an invaluable tool in recognizing unknown protein–ligand affinities.
<br/>来源：生物谷
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